Identification of QTLs for powdery mildew (Podosphaera aphanis; syn. Sphaerotheca macularis f. sp. fragariae) susceptibility in cultivated strawberry (Fragaria ×ananassa)

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Initiation, development, and survival of cleistothecia of Podosphaera aphanis and their role in the epidemiology of strawberry powdery mildew.

A collection of four clonal isolates of Podosphaera aphanis was heterothallic and was composed of two mutually exclusive mating types. Cleistothecial initials approximately 20 to 30 microm in diameter were observed within 7 to 14 days after pairing of compatible isolates and developed into morphologically mature ascocarps within 4 weeks after initiation on both potted plants maintained in isola...

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Transgene transmission frequencies between cultivated strawberry (Fragaria x ananassa Dutch) and other Fragaria species

The transmission of transgenes (uid-A and nptII) was studied in crosses between transgenic plants, with Fragaria x ananassa cultivar Teodora as the donor plant and various Fragaria species as recipient plants. GUS expression and kanamycin resistance were evaluated in embryos and in seedlings after aseptical germination, both in intraand inter-specific crosses as demonstrated by different ploidy...

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MICROPROPAGATION OF STRAWBERRY (Fragaria ananassa) THROUGH RUNNER CULTURE

In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was ob...

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Identification and Validation of Reference Genes for Transcript Normalization in Strawberry (Fragaria × ananassa) Defense Responses

Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are dependent upon the choice of an optimal reference control transcript. There is no information ava...

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Labor-saving Preservation of Powdery Mildew of Strawberry by Sterilized Seedling Culture

Various preservation methods at low temperature have been reported for several powdery mildew fungi, but the conidia of Podosphaera aphanis causing powdery mildew of strawberry could not be preserved either at low temperature or by freeze-drying and liquid-drying of conidial suspensions or diseased leaf discs. Here, we developed a new technique for a labor-saving preservation of the conidia of ...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2019

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0222829